{"id":"doi:10.1002/adma.202110054","kind":"article","title":"Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver-Like Metabolic Biofactories","authors":["Bernal PN","Bouwmeester M","Madrid-Wolff J","Falandt M","Florczak S","Rodriguez NG","Li Y","Größbacher G","Samsom RA","van Wolferen M","van der Laan LJW","Delrot P","Loterie D","Malda J","Moser C","Spee B","Levato R"],"author_count":17,"year":2022,"published":"2022-03-06","venue":"Advanced materials (Deerfield Beach, Fla.)","cited_by":206,"doi":"10.1002/adma.202110054","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1002/adma.202110054","archive_url":"https://space-kitty.com/papers/doi:10.1002/adma.202110054","updated":null,"pmid":"35166410","pmcid":null,"openalex_id":null,"pdf_url":null,"abstract":"Organ- and tissue-level biological functions are intimately linked to microscale cell-cell interactions and to the overarching tissue architecture. Together, biofabrication and organoid technologies offer the unique potential to engineer multi-scale living constructs, with cellular microenvironments formed by stem cell self-assembled structures embedded in customizable bioprinted geometries. This study introduces the volumetric bioprinting of complex organoid-laden constructs, which capture key functions of the human liver. Volumetric bioprinting via optical tomography shapes organoid-laden gelatin hydrogels into complex centimeter-scale 3D structures in under 20 s. Optically tuned bioresins enable refractive index matching of specific intracellular structures, countering the disruptive impact of cell-mediated light scattering on printing resolution. This layerless, nozzle-free technique poses no harmful mechanical stresses on organoids, resulting in superior viability and morphology preservation post-printing. Bioprinted organoids undergo hepatocytic differentiation showing albumin synthesis, liver-specific enzyme activity, and remarkably acquired native-like polarization. Organoids embedded within low stiffness gelatins (<2 kPa) are bioprinted into mathematically defined lattices with varying degrees of pore network tortuosity, and cultured under perfusion. These structures act as metabolic biofactories in which liver-specific ammonia detoxification can be enhanced by the architectural profile of the constructs. This technology opens up new possibilities for regenerative medicine and personalized drug testing.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":false,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver-Like Metabolic Biofactories\" by Bernal PN, Bouwmeester M, Madrid-Wolff J et al., https://doi.org/10.1002/adma.202110054, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"organoids","name":"Organoids & Organ-on-Chip","domain_slug":"biotech"}],"cited_in_dossiers":[]}