{"id":"doi:10.1002/cpcb.98","kind":"article","title":"Preparation and Co-Culture of iPSC-Derived Dopaminergic Neurons and Astrocytes","authors":["de Rus Jacquet A"],"author_count":1,"year":2019,"published":"2019-12-01","venue":"Current protocols in cell biology","cited_by":33,"doi":"10.1002/cpcb.98","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1002/cpcb.98","archive_url":"https://space-kitty.com/papers/doi:10.1002/cpcb.98","updated":null,"pmid":"31763766","pmcid":"PMC9285934","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC9285934/pdf","abstract":"Induced pluripotent stem cell (iPSC)-based models are powerful tools to study neurodegenerative diseases such as Parkinson's disease. The differentiation of patient-derived neurons and astrocytes allows investigation of the molecular mechanisms responsible for disease onset and development. In particular, these two cell types can be mono- or co-cultured to study the influence of cell-autonomous and non-cell-autonomous contributors to neurodegenerative diseases. We developed a streamlined procedure to produce high-quality/high-purity cultures of dopaminergic neurons and astrocytes that originate from the same population of midbrain floor-plate progenitors. This unit describes differentiation, quality control, culture parameters, and troubleshooting tips to ensure the highest quality and reproducibility of research results. © 2019 The Authors. Basic Protocol 1: Differentiation of iPSCs into midbrain-patterned neural progenitor cells Support Protocol: Quality control of neural progenitor cells Basic Protocol 2: Differentiation of neural progenitor cells into astrocytes Basic Protocol 3: Differentiation of neural progenitor cells into dopaminergic neurons Basic Protocol 4: Co-culture of iPSC-derived neurons and astrocytes.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"Preparation and Co-Culture of iPSC-Derived Dopaminergic Neurons and Astrocytes\" by de Rus Jacquet A, https://doi.org/10.1002/cpcb.98, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"gene-cell-therapy","name":"Gene & Cell Therapy","domain_slug":"medicine"}],"cited_in_dossiers":[]}