{"id":"doi:10.1016/j.cell.2022.11.005","kind":"article","title":"A human fetal lung cell atlas uncovers proximal-distal gradients of differentiation and key regulators of epithelial fates","authors":["He P","Lim K","Sun D","Pett JP","Jeng Q","Polanski K","Dong Z","Bolt L","Richardson L","Mamanova L","Dabrowska M","Wilbrey-Clark A","Madissoon E","Tuong ZK","Dann E","Suo C","Goh I","Yoshida M","Nikolić MZ","Janes SM","He X","Barker RA","Teichmann SA","Marioni JC","Meyer KB"],"author_count":26,"year":2022,"published":"2022-12-01","venue":"Cell","cited_by":209,"doi":"10.1016/j.cell.2022.11.005","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1016/j.cell.2022.11.005","archive_url":"https://space-kitty.com/papers/doi:10.1016/j.cell.2022.11.005","updated":null,"pmid":"36493756","pmcid":"PMC7618435","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC7618435/pdf","abstract":"We present a multiomic cell atlas of human lung development that combines single-cell RNA and ATAC sequencing, high-throughput spatial transcriptomics, and single-cell imaging. Coupling single-cell methods with spatial analysis has allowed a comprehensive cellular survey of the epithelial, mesenchymal, endothelial, and erythrocyte/leukocyte compartments from 5-22 post-conception weeks. We identify previously uncharacterized cell states in all compartments. These include developmental-specific secretory progenitors and a subtype of neuroendocrine cell related to human small cell lung cancer. Our datasets are available through our web interface (https://lungcellatlas.org). To illustrate its general utility, we use our cell atlas to generate predictions about cell-cell signaling and transcription factor hierarchies which we rigorously test using organoid models.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"A human fetal lung cell atlas uncovers proximal-distal gradients of differentiation and key regulators of epithelial fates\" by He P, Lim K, Sun D et al., https://doi.org/10.1016/j.cell.2022.11.005, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"single-cell-spatial","name":"Single-Cell & Spatial Omics","domain_slug":"biotech"},{"slug":"organoids","name":"Organoids & Organ-on-Chip","domain_slug":"biotech"}],"cited_in_dossiers":[]}