{"id":"doi:10.1016/j.ijregi.2026.100969","kind":"article","title":"Microbiological profiles of Gram-negative bacteria isolated from respiratory samples of mechanically ventilated patients at a tertiary Moroccan hospital","authors":["El Ftouh S","Tligui H","Vila J","Aberouch L","Dendane T","Oualili L","Ssouni O","Faroudy M","Zeggwagh AA","Doumiri M","Pitart C","Piquet M","Guiral E","Abidi K"],"author_count":14,"year":2026,"published":"2026-08-11","venue":"IJID regions","cited_by":0,"doi":"10.1016/j.ijregi.2026.100969","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1016/j.ijregi.2026.100969","archive_url":"https://space-kitty.com/papers/doi:10.1016/j.ijregi.2026.100969","updated":null,"pmid":"42824716","pmcid":"PMC13627934","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC13627934/pdf","abstract":"Objectives To characterise the Gram-negative bacteria implicated in ventilator-associated pneumonia (VAP), focusing on their antimicrobial resistance profiles and phylogenetic relationships. Methods Seventy-eight isolates were collected from protected distal samples over two years; patients were under mechanical ventilation in the three adult ICUs of a Moroccan tertiary hospital. Isolates were identified using VITEK2. PFGE and FTIR determined genetic relationships, whereas the detection of genetic resistance determinants was carried out using PCR. Results Carbapenem-resistant Acinetobacter baumannii harbouring the bla - OXA-23 was the predominant Gram-negative bacterium. Klebsiella pneumoniae was the most prevalent among the Enterobacterales, with a high resistance rate to almost all antibiotics, due to the coexistence of ( bla ) CTX-M-1, TEM, SHV , and OXA-48-like genes. Escherichia coli exhibited high resistance to ciprofloxacin (86%), ceftazidime (86%), and cotrimoxazole (71%). Proteus mirabilis was highly resistant to cotrimoxazole (60%) and ticarcillin (80%). Pseudomonas aeruginosa was resistant to carbapenems in 39% of cases, in which bla NDM and bla VIM were the only metallo-beta-lactamase genes detected. In phylogenetic analysis, both FTIR and PFGE techniques underscored no epidemiological relationship among strains. Conclusion The prevalence of MDR and XDR bacteria among VAP cases in a tertiary hospital in Morocco is increasing, potentially due to the coexistence of antimicrobial resistance genes.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"Microbiological profiles of Gram-negative bacteria isolated from respiratory samples of mechanically ventilated patients at a tertiary Moroccan hospital\" by El Ftouh S, Tligui H, Vila J et al., https://doi.org/10.1016/j.ijregi.2026.100969, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"infectious-disease-amr","name":"Infectious Disease, Vaccines & Antimicrobial Resistance","domain_slug":"medicine"}],"cited_in_dossiers":[]}