{"id":"doi:10.1016/j.isci.2020.101305","kind":"article","title":"Extended Metabolic Biosensor Design for Dynamic Pathway Regulation of Cell Factories","authors":["Boada Y","Vignoni A","Picó J","Carbonell P"],"author_count":4,"year":2020,"published":"2020-06-23","venue":"iScience","cited_by":40,"doi":"10.1016/j.isci.2020.101305","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1016/j.isci.2020.101305","archive_url":"https://space-kitty.com/papers/doi:10.1016/j.isci.2020.101305","updated":null,"pmid":"32629420","pmcid":"PMC7334618","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC7334618/pdf","abstract":"Transcription factor-based biosensors naturally occur in metabolic pathways to maintain cell growth and to provide a robust response to environmental fluctuations. Extended metabolic biosensors, i.e., the cascading of a bio-conversion pathway and a transcription factor (TF) responsive to the downstream effector metabolite, provide sensing capabilities beyond natural effectors for implementing context-aware synthetic genetic circuits and bio-observers. However, the engineering of such multi-step circuits is challenged by stability and robustness issues. In order to streamline the design of TF-based biosensors in metabolic pathways, here we investigate the response of a genetic circuit combining a TF-based extended metabolic biosensor with an antithetic integral circuit, a feedback controller that achieves robustness against environmental fluctuations. The dynamic response of an extended biosensor-based regulated flavonoid pathway is analyzed in order to address the issues of biosensor tuning of the regulated pathway under industrial biomanufacturing operating constraints.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"Extended Metabolic Biosensor Design for Dynamic Pathway Regulation of Cell Factories\" by Boada Y, Vignoni A, Picó J et al., https://doi.org/10.1016/j.isci.2020.101305, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"synthetic-biology","name":"Synthetic Biology & Genome Engineering","domain_slug":"biotech"}],"cited_in_dossiers":[]}