{"id":"doi:10.1016/j.omta.2026.201789","kind":"article","title":"Sindbis virus self-amplifying replicon is compatible with modified nucleotides mediating expression and vaccine responses <i>in vivo</i>","authors":["Azizi H","Agbayani G","Renner TM","Simard B","Iqbal U","Dudani R","Jia Y","McCluskie MJ","Akache B"],"author_count":9,"year":2026,"published":"2026-06-18","venue":"Molecular therapy. Advances","cited_by":0,"doi":"10.1016/j.omta.2026.201789","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1016/j.omta.2026.201789","archive_url":"https://space-kitty.com/papers/doi:10.1016/j.omta.2026.201789","updated":null,"pmid":"42436853","pmcid":"PMC13355167","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC13355167/pdf","abstract":"The advent of effective formulations based on RNA encapsulated within lipid nanoparticles has led to the evaluation of this technology for its ability to treat and prevent a wide range of diseases. Expansion of the RNA-based tool kit available to vaccine and drug developers will accelerate this development and hopefully produce more robust and cost-effective therapies. With its more sustained expression profile, self-amplifying RNA offers many advantages to standard messenger RNA, allowing for the administration of lower RNA doses in vivo and potentially reducing dosing frequency for indications where long-term expression of a protein is required. We generated self-amplifying RNA containing the viral replicon from Sindbis virus, which demonstrated robust protein expression in vitro and in vivo . In addition, it showed increased compatibility with a number of modified nucleotides when compared to an equivalent RNA containing the replicon from Venezuelan equine encephalitis virus. These Sindbis-based self-amplifying RNAs containing modified nucleotides have the potential to be used in a variety of experimental and therapeutic applications.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"Sindbis virus self-amplifying replicon is compatible with modified nucleotides mediating expression and vaccine responses <i>in vivo</i>\" by Azizi H, Agbayani G, Renner TM et al., https://doi.org/10.1016/j.omta.2026.201789, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"mrna-cancer-vaccines","name":"mRNA Therapeutics & Cancer Vaccines","domain_slug":"medicine"}],"cited_in_dossiers":[]}