{"id":"doi:10.1016/j.semcancer.2018.04.001","kind":"review","title":"CRISPR/Cas9 for cancer research and therapy","authors":["Zhan T","Rindtorff N","Betge J","Ebert MP","Boutros M"],"author_count":5,"year":2019,"published":"2018-04-16","venue":"Seminars in cancer biology","cited_by":216,"doi":"10.1016/j.semcancer.2018.04.001","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1016/j.semcancer.2018.04.001","archive_url":"https://space-kitty.com/papers/doi:10.1016/j.semcancer.2018.04.001","updated":null,"pmid":"29673923","pmcid":null,"openalex_id":null,"pdf_url":null,"abstract":"CRISPR/Cas9 has become a powerful method for making changes to the genome of many organisms. First discovered in bacteria as part of an adaptive immune system, CRISPR/Cas9 and modified versions have found a widespread use to engineer genomes and to activate or to repress the expression of genes. As such, CRISPR/Cas9 promises to accelerate cancer research by providing an efficient technology to dissect mechanisms of tumorigenesis, identify targets for drug development, and possibly arm cells for cell-based therapies. Here, we review current applications of the CRISPR/Cas9 technology for cancer research and therapy. We describe novel Cas9 variants and how they are used in functional genomics to discover novel cancer-specific vulnerabilities. Furthermore, we highlight the impact of CRISPR/Cas9 in generating organoid and mouse models of cancer. Finally, we provide an overview of the first clinical trials that apply CRISPR/Cas9 as a therapeutic approach against cancer.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":false,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"CRISPR/Cas9 for cancer research and therapy\" by Zhan T, Rindtorff N, Betge J et al., https://doi.org/10.1016/j.semcancer.2018.04.001, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"organoids","name":"Organoids & Organ-on-Chip","domain_slug":"biotech"}],"cited_in_dossiers":[]}