{"id":"doi:10.1038/ncomms6392","kind":"article","title":"Mind-controlled transgene expression by a wireless-powered optogenetic designer cell implant","authors":["Folcher M","Oesterle S","Zwicky K","Thekkottil T","Heymoz J","Hohmann M","Christen M","Daoud El-Baba M","Buchmann P","Fussenegger M"],"author_count":10,"year":2014,"published":"2014-11-11","venue":"Nature communications","cited_by":89,"doi":"10.1038/ncomms6392","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1038/ncomms6392","archive_url":"https://space-kitty.com/papers/doi:10.1038/ncomms6392","updated":null,"pmid":"25386727","pmcid":"PMC4241983","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC4241983/pdf","abstract":"Synthetic devices for traceless remote control of gene expression may provide new treatment opportunities in future gene- and cell-based therapies. Here we report the design of a synthetic mind-controlled gene switch that enables human brain activities and mental states to wirelessly programme the transgene expression in human cells. An electroencephalography (EEG)-based brain-computer interface (BCI) processing mental state-specific brain waves programs an inductively linked wireless-powered optogenetic implant containing designer cells engineered for near-infrared (NIR) light-adjustable expression of the human glycoprotein SEAP (secreted alkaline phosphatase). The synthetic optogenetic signalling pathway interfacing the BCI with target gene expression consists of an engineered NIR light-activated bacterial diguanylate cyclase (DGCL) producing the orthogonal second messenger cyclic diguanosine monophosphate (c-di-GMP), which triggers the stimulator of interferon genes (STING)-dependent induction of synthetic interferon-β promoters. Humans generating different mental states (biofeedback control, concentration, meditation) can differentially control SEAP production of the designer cells in culture and of subcutaneous wireless-powered optogenetic implants in mice.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"Mind-controlled transgene expression by a wireless-powered optogenetic designer cell implant\" by Folcher M, Oesterle S, Zwicky K et al., https://doi.org/10.1038/ncomms6392, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"brain-computer-interfaces","name":"Brain-Computer Interfaces","domain_slug":"neuroscience"}],"cited_in_dossiers":[]}