{"id":"doi:10.1038/ncomms8923","kind":"article","title":"Large-volume en-bloc staining for electron microscopy-based connectomics","authors":["Hua Y","Laserstein P","Helmstaedter M"],"author_count":3,"year":2015,"published":"2015-08-03","venue":"Nature communications","cited_by":255,"doi":"10.1038/ncomms8923","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1038/ncomms8923","archive_url":"https://space-kitty.com/papers/doi:10.1038/ncomms8923","updated":null,"pmid":"26235643","pmcid":"PMC4532871","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC4532871/pdf","abstract":"Large-scale connectomics requires dense staining of neuronal tissue blocks for electron microscopy (EM). Here we report a large-volume dense en-bloc EM staining protocol that overcomes the staining gradients, which so far substantially limited the reconstructable volumes in three-dimensional (3D) EM. Our protocol provides densely reconstructable tissue blocks from mouse neocortex sized at least 1 mm in diameter. By relaxing the constraints on precise topographic sample targeting, it makes the correlated functional and structural analysis of neuronal circuits realistic.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"Large-volume en-bloc staining for electron microscopy-based connectomics\" by Hua Y, Laserstein P, Helmstaedter M, https://doi.org/10.1038/ncomms8923, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"connectomics","name":"Connectomics & Brain Mapping","domain_slug":"neuroscience"}],"cited_in_dossiers":[]}