{"id":"doi:10.1038/s41426-018-0124-z","kind":"article","title":"Emergence of a novel mobile colistin resistance gene, mcr-8 , in NDM-producing Klebsiella pneumoniae","authors":["Xiaoming Wang","Yao Wang","Ying Zhou","Jiyun Li","Wenjuan Yin","Shaolin Wang","Suxia Zhang","Jianzhong Shen","Zhangqi Shen","Yang Wang"],"author_count":10,"year":2018,"published":"2018-06-30","venue":"Emerging Microbes & Infections","cited_by":461,"doi":"10.1038/s41426-018-0124-z","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1038/s41426-018-0124-z","archive_url":"https://space-kitty.com/papers/doi:10.1038/s41426-018-0124-z","updated":null,"pmid":"29970891","pmcid":"PMC6030107","openalex_id":"W2810427827","pdf_url":"https://www.tandfonline.com/doi/pdf/10.1038/s41426-018-0124-z?needAccess=true","abstract":"The rapid increase in carbapenem resistance among gram-negative bacteria has renewed focus on the importance of polymyxin antibiotics (colistin or polymyxin E). However, the recent emergence of plasmid-mediated colistin resistance determinants (mcr-1, -2, -3, -4, -5, -6, and -7), especially mcr-1, in carbapenem-resistant Enterobacteriaceae is a serious threat to global health. Here, we characterized a novel mobile colistin resistance gene, mcr-8, located on a transferrable 95,983-bp IncFII-type plasmid in Klebsiella pneumoniae. The deduced amino-acid sequence of MCR-8 showed 31.08%, 30.26%, 39.96%, 37.85%, 33.51%, 30.43%, and 37.46% identity to MCR-1, MCR-2, MCR-3, MCR-4, MCR-5, MCR-6, and MCR-7, respectively. Functional cloning indicated that the acquisition of the single mcr-8 gene significantly increased resistance to colistin in both Escherichia coli and K. pneumoniae. Notably, the coexistence of mcr-8 and the carbapenemase-encoding gene blaNDM was confirmed in K. pneumoniae isolates of livestock origin. Moreover, BLASTn analysis of mcr-8 revealed that this gene was present in a colistin- and carbapenem-resistant K. pneumoniae strain isolated from the sputum of a patient with pneumonia syndrome in the respiratory intensive care unit of a Chinese hospital in 2016. These findings indicated that mcr-8 has existed for some time and has disseminated among K. pneumoniae of both animal and human origin, further increasing the public health burden of antimicrobial resistance.","abstract_license":"CC-BY (publisher license, via OpenAlex)","license":"cc-by","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":["Antibiotic Resistance in Bacteria"],"details":null,"provenance":"openalex+europepmc","attribution":"Abstract of \"Emergence of a novel mobile colistin resistance gene, mcr-8 , in NDM-producing Klebsiella pneumoniae\" by Xiaoming Wang, Yao Wang, Ying Zhou et al., https://doi.org/10.1038/s41426-018-0124-z, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"infectious-disease-amr","name":"Infectious Disease, Vaccines & Antimicrobial Resistance","domain_slug":"medicine"}],"cited_in_dossiers":[]}