{"id":"doi:10.1038/s41467-017-00259-3","kind":"article","title":"Membrane-free culture and real-time barrier integrity assessment of perfused intestinal epithelium tubes","authors":["Trietsch SJ","Naumovska E","Kurek D","Setyawati MC","Vormann MK","Wilschut KJ","Lanz HL","Nicolas A","Ng CP","Joore J","Kustermann S","Roth A","Hankemeier T","Moisan A","Vulto P"],"author_count":15,"year":2017,"published":"2017-08-15","venue":"Nature communications","cited_by":234,"doi":"10.1038/s41467-017-00259-3","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1038/s41467-017-00259-3","archive_url":"https://space-kitty.com/papers/doi:10.1038/s41467-017-00259-3","updated":null,"pmid":"28811479","pmcid":"PMC5557798","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC5557798/pdf","abstract":"In vitro models that better reflect in vivo epithelial barrier (patho-)physiology are urgently required to predict adverse drug effects. Here we introduce extracellular matrix-supported intestinal tubules in perfused microfluidic devices, exhibiting tissue polarization and transporter expression. Forty leak-tight tubules are cultured in parallel on a single plate and their response to pharmacological stimuli is recorded over 125 h using automated imaging techniques. A study comprising 357 gut tubes is performed, of which 93% are leak tight before exposure. EC 50 -time curves could be extracted that provide insight into both concentration and exposure time response. Full compatibility with standard equipment and user-friendly operation make this Organ-on-a-Chip platform readily applicable in routine laboratories.Efforts to determine the effects of drugs on epithelial barriers could benefit from better in vitro models. Here the authors develop a microfluidic device supporting the growth and function of extracellular matrix-supported intestinal tubules, and evaluate the effect of staurosporine and acetylsalicylic acid on barrier integrity.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"Membrane-free culture and real-time barrier integrity assessment of perfused intestinal epithelium tubes\" by Trietsch SJ, Naumovska E, Kurek D et al., https://doi.org/10.1038/s41467-017-00259-3, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"organoids","name":"Organoids & Organ-on-Chip","domain_slug":"biotech"}],"cited_in_dossiers":[]}