{"id":"doi:10.1038/s41467-017-02473-5","kind":"article","title":"Tuning the dynamic range of bacterial promoters regulated by ligand-inducible transcription factors","authors":["Chen Y","Ho JML","Shis DL","Gupta C","Long J","Wagner DS","Ott W","Josić K","Bennett MR"],"author_count":9,"year":2018,"published":"2018-01-04","venue":"Nature communications","cited_by":148,"doi":"10.1038/s41467-017-02473-5","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1038/s41467-017-02473-5","archive_url":"https://space-kitty.com/papers/doi:10.1038/s41467-017-02473-5","updated":null,"pmid":"29302024","pmcid":"PMC5754348","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC5754348/pdf","abstract":"One challenge for synthetic biologists is the predictable tuning of genetic circuit regulatory components to elicit desired outputs. Gene expression driven by ligand-inducible transcription factor systems must exhibit the correct ON and OFF characteristics: appropriate activation and leakiness in the presence and absence of inducer, respectively. However, the dynamic range of a promoter (i.e., absolute difference between ON and OFF states) is difficult to control. We report a method that tunes the dynamic range of ligand-inducible promoters to achieve desired ON and OFF characteristics. We build combinatorial sets of AraC-and LasR-regulated promoters containing -10 and -35 sites from synthetic and Escherichia coli promoters. Four sequence combinations with diverse dynamic ranges were chosen to build multi-input transcriptional logic gates regulated by two and three ligand-inducible transcription factors (LacI, TetR, AraC, XylS, RhlR, LasR, and LuxR). This work enables predictable control over the dynamic range of regulatory components.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"Tuning the dynamic range of bacterial promoters regulated by ligand-inducible transcription factors\" by Chen Y, Ho JML, Shis DL et al., https://doi.org/10.1038/s41467-017-02473-5, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"synthetic-biology","name":"Synthetic Biology & Genome Engineering","domain_slug":"biotech"}],"cited_in_dossiers":[]}