{"id":"doi:10.1038/s41467-018-05915-w","kind":"article","title":"Engineered bidirectional promoters enable rapid multi-gene co-expression optimization","authors":["Thomas Vogl","Thomas Kickenweiz","Julia Pitzer","Lukas Sturmberger","Astrid Weninger","Bradley Walters Biggs","Eva-Maria Köhler","Armin Baumschlager","Jasmin Elgin Fischer","Patrick Hyden","Marlies Wagner","Martina Baumann","Nicole Borth","Martina Geier","Parayil Kumaran Ajikumar","Anton Glieder"],"author_count":16,"year":2018,"published":"2018-08-29","venue":"Nature Communications","cited_by":139,"doi":"10.1038/s41467-018-05915-w","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1038/s41467-018-05915-w","archive_url":"https://space-kitty.com/papers/doi:10.1038/s41467-018-05915-w","updated":null,"pmid":"30181586","pmcid":"PMC6123417","openalex_id":"W2888847278","pdf_url":"https://www.nature.com/articles/s41467-018-05915-w.pdf","abstract":"Numerous synthetic biology endeavors require well-tuned co-expression of functional components for success. Classically, monodirectional promoters (MDPs) have been used for such applications, but MDPs are limited in terms of multi-gene co-expression capabilities. Consequently, there is a pressing need for new tools with improved flexibility in terms of genetic circuit design, metabolic pathway assembly, and optimization. Here, motivated by nature's use of bidirectional promoters (BDPs) as a solution for efficient gene co-expression, we generate a library of 168 synthetic BDPs in the yeast Komagataella phaffii (syn. Pichia pastoris), leveraging naturally occurring BDPs as a parts repository. This library of synthetic BDPs allows for rapid screening of diverse expression profiles and ratios to optimize gene co-expression, including for metabolic pathways (taxadiene, β-carotene). The modular design strategies applied for creating the BDP library could be relevant in other eukaryotic hosts, enabling a myriad of metabolic engineering and synthetic biology applications.","abstract_license":"CC-BY (publisher license, via OpenAlex)","license":"cc-by","is_open_access":true,"peer_reviewed":null,"categories":["Microbial Metabolic Engineering and Bioproduction"],"details":null,"provenance":"openalex+europepmc","attribution":"Abstract of \"Engineered bidirectional promoters enable rapid multi-gene co-expression optimization\" by Thomas Vogl, Thomas Kickenweiz, Julia Pitzer et al., https://doi.org/10.1038/s41467-018-05915-w, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"synthetic-biology","name":"Synthetic Biology & Genome Engineering","domain_slug":"biotech"}],"cited_in_dossiers":[]}