{"id":"doi:10.1038/s41467-020-17409-9","kind":"article","title":"Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine candidate induces high neutralizing antibody titers in mice","authors":["McKay PF","Hu K","Blakney AK","Samnuan K","Brown JC","Penn R","Zhou J","Bouton CR","Rogers P","Polra K","Lin PJC","Barbosa C","Tam YK","Barclay WS","Shattock RJ"],"author_count":15,"year":2020,"published":"2020-07-09","venue":"Nature communications","cited_by":378,"doi":"10.1038/s41467-020-17409-9","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1038/s41467-020-17409-9","archive_url":"https://space-kitty.com/papers/doi:10.1038/s41467-020-17409-9","updated":null,"pmid":"32647131","pmcid":"PMC7347890","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC7347890/pdf","abstract":"The spread of the SARS-CoV-2 into a global pandemic within a few months of onset motivates the development of a rapidly scalable vaccine. Here, we present a self-amplifying RNA encoding the SARS-CoV-2 spike protein encapsulated within a lipid nanoparticle (LNP) as a vaccine. We observe remarkably high and dose-dependent SARS-CoV-2 specific antibody titers in mouse sera, as well as robust neutralization of both a pseudo-virus and wild-type virus. Upon further characterization we find that the neutralization is proportional to the quantity of specific IgG and of higher magnitude than recovered COVID-19 patients. saRNA LNP immunizations induce a Th1-biased response in mice, and there is no antibody-dependent enhancement (ADE) observed. Finally, we observe high cellular responses, as characterized by IFN-γ production, upon re-stimulation with SARS-CoV-2 peptides. These data provide insight into the vaccine design and evaluation of immunogenicity to enable rapid translation to the clinic.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine candidate induces high neutralizing antibody titers in mice\" by McKay PF, Hu K, Blakney AK et al., https://doi.org/10.1038/s41467-020-17409-9, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"mrna-cancer-vaccines","name":"mRNA Therapeutics & Cancer Vaccines","domain_slug":"medicine"}],"cited_in_dossiers":[]}