{"id":"doi:10.1038/s41467-021-21337-7","kind":"article","title":"PrimeDesign software for rapid and simplified design of prime editing guide RNAs","authors":["Hsu JY","Grünewald J","Szalay R","Shih J","Anzalone AV","Lam KC","Shen MW","Petri K","Liu DR","Joung JK","Pinello L"],"author_count":11,"year":2021,"published":"2021-02-15","venue":"Nature communications","cited_by":153,"doi":"10.1038/s41467-021-21337-7","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1038/s41467-021-21337-7","archive_url":"https://space-kitty.com/papers/doi:10.1038/s41467-021-21337-7","updated":null,"pmid":"33589617","pmcid":"PMC7884779","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC7884779/pdf","abstract":"Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web application and command-line tool for the design of PE experiments. PrimeDesign can be used for single and combination editing applications, as well as genome-wide and saturation mutagenesis screens. Using PrimeDesign, we construct PrimeVar, a comprehensive and searchable database that includes candidate prime editing guide RNA (pegRNA) and nicking sgRNA (ngRNA) combinations for installing or correcting >68,500 pathogenic human genetic variants from the ClinVar database. Finally, we use PrimeDesign to design pegRNAs/ngRNAs to install a variety of human pathogenic variants in human cells.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"PrimeDesign software for rapid and simplified design of prime editing guide RNAs\" by Hsu JY, Grünewald J, Szalay R et al., https://doi.org/10.1038/s41467-021-21337-7, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"gene-editing-therapies","name":"CRISPR & Gene-Editing Therapies","domain_slug":"medicine"}],"cited_in_dossiers":[]}