{"id":"doi:10.1038/s41467-022-29507-x","kind":"article","title":"Enhancement of prime editing via xrRNA motif-joined pegRNA","authors":["Zhang G","Liu Y","Huang S","Qu S","Cheng D","Yao Y","Ji Q","Wang X","Huang X","Liu J"],"author_count":10,"year":2022,"published":"2022-04-06","venue":"Nature communications","cited_by":96,"doi":"10.1038/s41467-022-29507-x","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1038/s41467-022-29507-x","archive_url":"https://space-kitty.com/papers/doi:10.1038/s41467-022-29507-x","updated":null,"pmid":"35387980","pmcid":"PMC8986804","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC8986804/pdf","abstract":"The prime editors (PEs) have shown great promise for precise genome modification. However, their suboptimal efficiencies present a significant technical challenge. Here, by appending a viral exoribonuclease-resistant RNA motif (xrRNA) to the 3'-extended portion of pegRNAs for their increased resistance against degradation, we develop an upgraded PE platform (xrPE) with substantially enhanced editing efficiencies in multiple cell lines. A pan-target average enhancement of up to 3.1-, 4.5- and 2.5-fold in given cell types is observed for base conversions, small deletions, and small insertions, respectively. Additionally, xrPE exhibits comparable edit:indel ratios and similarly minimal off-target editing as the canonical PE3. Of note, parallel comparison of xrPE to the most recently developed epegRNA-based PE system shows their largely equivalent editing performances. Our study establishes a highly adaptable platform of improved PE that shall have broad implications.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"Enhancement of prime editing via xrRNA motif-joined pegRNA\" by Zhang G, Liu Y, Huang S et al., https://doi.org/10.1038/s41467-022-29507-x, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"gene-editing-therapies","name":"CRISPR & Gene-Editing Therapies","domain_slug":"medicine"}],"cited_in_dossiers":[]}