{"id":"doi:10.1038/s41467-022-32284-2","kind":"article","title":"Spatiotemporal dynamics of macrophage heterogeneity and a potential function of Trem2 hi macrophages in infarcted hearts","authors":["Jung SH","Hwang BH","Shin S","Park EH","Park SH","Kim CW","Kim E","Choo E","Choi IJ","Swirski FK","Chang K","Chung YJ"],"author_count":12,"year":2022,"published":"2022-08-06","venue":"Nature communications","cited_by":195,"doi":"10.1038/s41467-022-32284-2","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1038/s41467-022-32284-2","archive_url":"https://space-kitty.com/papers/doi:10.1038/s41467-022-32284-2","updated":null,"pmid":"35933399","pmcid":"PMC9357004","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC9357004/pdf","abstract":"Heart failure (HF) is a frequent consequence of myocardial infarction (MI). Identification of the precise, time-dependent composition of inflammatory cells may provide clues for the establishment of new biomarkers and therapeutic approaches targeting post-MI HF. Here, we investigate the spatiotemporal dynamics of MI-associated immune cells in a mouse model of MI using spatial transcriptomics and single-cell RNA-sequencing (scRNA-seq). We identify twelve major immune cell populations; their proportions dynamically change after MI. Macrophages are the most abundant population at all-time points (>60%), except for day 1 post-MI. Trajectory inference analysis shows upregulation of Trem2 expression in macrophages during the late phase post-MI. In vivo injection of soluble Trem2 leads to significant functional and structural improvements in infarcted hearts. Our data contribute to a better understanding of MI-driven immune responses and further investigation to determine the regulatory factors of the Trem2 signaling pathway will aid the development of novel therapeutic strategies for post-MI HF.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"Spatiotemporal dynamics of macrophage heterogeneity and a potential function of Trem2 hi macrophages in infarcted hearts\" by Jung SH, Hwang BH, Shin S et al., https://doi.org/10.1038/s41467-022-32284-2, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"single-cell-spatial","name":"Single-Cell & Spatial Omics","domain_slug":"biotech"}],"cited_in_dossiers":[]}