{"id":"doi:10.1038/s41467-023-38457-x","kind":"article","title":"saRNA vaccine expressing membrane-anchored RBD elicits broad and durable immunity against SARS-CoV-2 variants of concern","authors":["Komori M","Nogimori T","Morey AL","Sekida T","Ishimoto K","Hassett MR","Masuta Y","Ode H","Tamura T","Suzuki R","Alexander J","Kido Y","Matsuda K","Fukuhara T","Iwatani Y","Yamamoto T","Smith JF","Akahata W"],"author_count":18,"year":2023,"published":"2023-05-19","venue":"Nature communications","cited_by":30,"doi":"10.1038/s41467-023-38457-x","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1038/s41467-023-38457-x","archive_url":"https://space-kitty.com/papers/doi:10.1038/s41467-023-38457-x","updated":null,"pmid":"37208330","pmcid":"PMC10199074","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC10199074/pdf","abstract":"Several vaccines have been widely used to counteract the global pandemic caused by SARS-CoV-2. However, due to the rapid emergence of SARS-CoV-2 variants of concern (VOCs), further development of vaccines that confer broad and longer-lasting protection against emerging VOCs are needed. Here, we report the immunological characteristics of a self-amplifying RNA (saRNA) vaccine expressing the SARS-CoV-2 Spike (S) receptor binding domain (RBD), which is membrane-anchored by fusing with an N-terminal signal sequence and a C-terminal transmembrane domain (RBD-TM). Immunization with saRNA RBD-TM delivered in lipid nanoparticles (LNP) efficiently induces T-cell and B-cell responses in non-human primates (NHPs). In addition, immunized hamsters and NHPs are protected against SARS-CoV-2 challenge. Importantly, RBD-specific antibodies against VOCs are maintained for at least 12 months in NHPs. These findings suggest that this saRNA platform expressing RBD-TM will be a useful vaccine candidate inducing durable immunity against emerging SARS-CoV-2 strains.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"saRNA vaccine expressing membrane-anchored RBD elicits broad and durable immunity against SARS-CoV-2 variants of concern\" by Komori M, Nogimori T, Morey AL et al., https://doi.org/10.1038/s41467-023-38457-x, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"mrna-cancer-vaccines","name":"mRNA Therapeutics & Cancer Vaccines","domain_slug":"medicine"}],"cited_in_dossiers":[]}