{"id":"doi:10.1038/s41467-023-38965-w","kind":"article","title":"A rapid cell-free expression and screening platform for antibody discovery","authors":["Hunt AC","Vögeli B","Hassan AO","Guerrero L","Kightlinger W","Yoesep DJ","Krüger A","DeWinter M","Diamond MS","Karim AS","Jewett MC"],"author_count":11,"year":2023,"published":"2023-07-03","venue":"Nature communications","cited_by":74,"doi":"10.1038/s41467-023-38965-w","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1038/s41467-023-38965-w","archive_url":"https://space-kitty.com/papers/doi:10.1038/s41467-023-38965-w","updated":null,"pmid":"37400446","pmcid":"PMC10318062","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC10318062/pdf","abstract":"Antibody discovery is bottlenecked by the individual expression and evaluation of antigen-specific hits. Here, we address this bottleneck by developing a workflow combining cell-free DNA template generation, cell-free protein synthesis, and binding measurements of antibody fragments in a process that takes hours rather than weeks. We apply this workflow to evaluate 135 previously published antibodies targeting the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including all 8 antibodies previously granted emergency use authorization for coronavirus disease 2019 (COVID-19), and demonstrate identification of the most potent antibodies. We also evaluate 119 anti-SARS-CoV-2 antibodies from a mouse immunized with the SARS-CoV-2 spike protein and identify neutralizing antibody candidates, including the antibody SC2-3, which binds the SARS-CoV-2 spike protein of all tested variants of concern. We expect that our cell-free workflow will accelerate the discovery and characterization of antibodies for future pandemics and for research, diagnostic, and therapeutic applications more broadly.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"A rapid cell-free expression and screening platform for antibody discovery\" by Hunt AC, Vögeli B, Hassan AO et al., https://doi.org/10.1038/s41467-023-38965-w, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"synthetic-biology","name":"Synthetic Biology & Genome Engineering","domain_slug":"biotech"}],"cited_in_dossiers":[]}