{"id":"doi:10.1038/s41551-022-00850-0","kind":"article","title":"Field validation of the performance of paper-based tests for the detection of the Zika and chikungunya viruses in serum samples","authors":["Karlikow M","da Silva SJR","Guo Y","Cicek S","Krokovsky L","Homme P","Xiong Y","Xu T","Calderón-Peláez MA","Camacho-Ortega S","Ma D","de Magalhães JJF","Souza BNRF","de Albuquerque Cabral DG","Jaenes K","Sutyrina P","Ferrante T","Benitez AD","Nipaz V","Ponce P","Rackus DG","Collins JJ","Paiva M","Castellanos JE","Cevallos V"],"author_count":29,"year":2022,"published":"2022-03-07","venue":"Nature biomedical engineering","cited_by":71,"doi":"10.1038/s41551-022-00850-0","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1038/s41551-022-00850-0","archive_url":"https://space-kitty.com/papers/doi:10.1038/s41551-022-00850-0","updated":null,"pmid":"35256758","pmcid":"PMC8940623","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC8940623/pdf","abstract":"In low-resource settings, resilience to infectious disease outbreaks can be hindered by limited access to diagnostic tests. Here we report the results of double-blinded studies of the performance of paper-based diagnostic tests for the Zika and chikungunya viruses in a field setting in Latin America. The tests involved a cell-free expression system relying on isothermal amplification and toehold-switch reactions, a purpose-built portable reader and onboard software for computer vision-enabled image analysis. In patients suspected of infection, the accuracies and sensitivities of the tests for the Zika and chikungunya viruses were, respectively, 98.5% (95% confidence interval, 96.2-99.6%, 268 serum samples) and 98.5% (95% confidence interval, 91.7-100%, 65 serum samples) and approximately 2 aM and 5 fM (both concentrations are within clinically relevant ranges). The analytical specificities and sensitivities of the tests for cultured samples of the viruses were equivalent to those of the real-time quantitative PCR. Cell-free synthetic biology tools and companion hardware can provide de-centralized, high-capacity and low-cost diagnostics for use in low-resource settings.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"Field validation of the performance of paper-based tests for the detection of the Zika and chikungunya viruses in serum samples\" by Karlikow M, da Silva SJR, Guo Y et al., https://doi.org/10.1038/s41551-022-00850-0, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"synthetic-biology","name":"Synthetic Biology & Genome Engineering","domain_slug":"biotech"}],"cited_in_dossiers":[]}