{"id":"doi:10.1038/s41588-022-01243-4","kind":"article","title":"A spatially resolved atlas of the human lung characterizes a gland-associated immune niche","authors":["Madissoon E","Oliver AJ","Kleshchevnikov V","Wilbrey-Clark A","Polanski K","Richoz N","Ribeiro Orsi A","Mamanova L","Bolt L","Elmentaite R","Pett JP","Huang N","Xu C","He P","Dabrowska M","Pritchard S","Tuck L","Prigmore E","Perera S","Knights A","Oszlanczi A","Hunter A","Vieira SF","Patel M","Lindeboom RGH"],"author_count":40,"year":2023,"published":"2022-12-21","venue":"Nature genetics","cited_by":211,"doi":"10.1038/s41588-022-01243-4","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1038/s41588-022-01243-4","archive_url":"https://space-kitty.com/papers/doi:10.1038/s41588-022-01243-4","updated":null,"pmid":"36543915","pmcid":"PMC9839452","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC9839452/pdf","abstract":"Single-cell transcriptomics has allowed unprecedented resolution of cell types/states in the human lung, but their spatial context is less well defined. To (re)define tissue architecture of lung and airways, we profiled five proximal-to-distal locations of healthy human lungs in depth using multi-omic single cell/nuclei and spatial transcriptomics (queryable at lungcellatlas.org ). Using computational data integration and analysis, we extend beyond the suspension cell paradigm and discover macro and micro-anatomical tissue compartments including previously unannotated cell types in the epithelial, vascular, stromal and nerve bundle micro-environments. We identify and implicate peribronchial fibroblasts in lung disease. Importantly, we discover and validate a survival niche for IgA plasma cells in the airway submucosal glands (SMG). We show that gland epithelial cells recruit B cells and IgA plasma cells, and promote longevity and antibody secretion locally through expression of CCL28, APRIL and IL-6. This new 'gland-associated immune niche' has implications for respiratory health.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"A spatially resolved atlas of the human lung characterizes a gland-associated immune niche\" by Madissoon E, Oliver AJ, Kleshchevnikov V et al., https://doi.org/10.1038/s41588-022-01243-4, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"single-cell-spatial","name":"Single-Cell & Spatial Omics","domain_slug":"biotech"}],"cited_in_dossiers":[]}