{"id":"doi:10.1038/s41588-024-01873-w","kind":"article","title":"An integrated single-cell reference atlas of the human endometrium","authors":["Marečková M","Garcia-Alonso L","Moullet M","Lorenzi V","Petryszak R","Sancho-Serra C","Oszlanczi A","Icoresi Mazzeo C","Wong FCK","Kelava I","Hoffman S","Krassowski M","Garbutt K","Gaitskell K","Yancheva S","Woon EV","Male V","Granne I","Hellner K","Mahbubani KT","Saeb-Parsy K","Lotfollahi M","Prigmore E","Southcombe J","Dragovic RA"],"author_count":28,"year":2024,"published":"2024-08-28","venue":"Nature genetics","cited_by":183,"doi":"10.1038/s41588-024-01873-w","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1038/s41588-024-01873-w","archive_url":"https://space-kitty.com/papers/doi:10.1038/s41588-024-01873-w","updated":null,"pmid":"39198675","pmcid":"PMC11387200","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC11387200/pdf","abstract":"The complex and dynamic cellular composition of the human endometrium remains poorly understood. Previous endometrial single-cell atlases profiled few donors and lacked consensus in defining cell types. We introduce the Human Endometrial Cell Atlas (HECA), a high-resolution single-cell reference atlas (313,527 cells) combining published and new endometrial single-cell transcriptomics datasets of 63 women with and without endometriosis. HECA assigns consensus and identifies previously unreported cell types, mapped in situ using spatial transcriptomics and validated using a new independent single-nuclei dataset (312,246 nuclei, 63 donors). In the functionalis, we identify intricate stromal-epithelial cell coordination via transforming growth factor beta (TGFβ) signaling. In the basalis, we define signaling between fibroblasts and an epithelial population expressing progenitor markers. Integration of HECA with large-scale endometriosis genome-wide association study data pinpoints decidualized stromal cells and macrophages as most likely dysregulated in endometriosis. The HECA is a valuable resource for studying endometrial physiology and disorders, and for guiding microphysiological in vitro systems development.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"An integrated single-cell reference atlas of the human endometrium\" by Marečková M, Garcia-Alonso L, Moullet M et al., https://doi.org/10.1038/s41588-024-01873-w, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"single-cell-spatial","name":"Single-Cell & Spatial Omics","domain_slug":"biotech"}],"cited_in_dossiers":[]}