{"id":"doi:10.1038/s41593-019-0431-2","kind":"article","title":"Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans","authors":["Josephine Jüttner","Arnold Szabó","Brigitte Gross Scherf","Rei Morikawa","Santiago B. Rompani","Miguel Teixeira","Péter Hantz","Tamás Szikra","Federico Esposti","Cameron S. Cowan","Arjun Bharioke","Claudia P. Patino-Alvarez","Özkan Keles","Chiara N. Roth","Ákos Kusnyerik","Nadine Gerber-Hollbach","Thierry Azoulay","Dominik Hartl","Arnaud Regis Krebs","Dirk Schübeler","Rozina Hajdu","Ákos Lukáts","János Németh","Zoltan Z. Nagy","Kun-Chao Wu"],"author_count":34,"year":2019,"published":"2019-07-08","venue":"Nature Neuroscience","cited_by":218,"doi":"10.1038/s41593-019-0431-2","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1038/s41593-019-0431-2","archive_url":"https://space-kitty.com/papers/doi:10.1038/s41593-019-0431-2","updated":null,"pmid":"31285614","pmcid":null,"openalex_id":"W2956118984","pdf_url":null,"abstract":"Summary Targeting genes to specific neuronal or glial cell types is valuable both for understanding and for repairing brain circuits. Adeno-associated viral vectors (AAVs) are frequently used for gene delivery, but targeting expression to specific cell types is a challenge. We created a library of 230 AAVs, each with a different synthetic promoter designed using four independent strategies. We show that ~11% of these AAVs specifically target expression to neuronal and glial cell types in the mouse retina, mouse brain, non-human primate retina in vivo , and in the human retina in vitro . We demonstrate applications for recording, stimulation, and molecular characterization, as well as the intersectional and combinatorial labeling of cell types. These resources and approaches allow economic, fast, and efficient cell-type targeting in a variety of species, both for fundamental science and for gene therapy.","abstract_license":"CC-BY (publisher license, via OpenAlex)","license":"cc-by","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":["Virus-based gene therapy research"],"details":null,"provenance":"openalex","attribution":"Abstract of \"Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans\" by Josephine Jüttner, Arnold Szabó, Brigitte Gross Scherf et al., https://doi.org/10.1038/s41593-019-0431-2, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"gene-cell-therapy","name":"Gene & Cell Therapy","domain_slug":"medicine"}],"cited_in_dossiers":[]}