{"id":"doi:10.1080/19490976.2026.2630483","kind":"article","title":"<b>Responses of intestinal organoids to infection by</b> <i><b>Mycobacterium avium</b></i> <b>resemble symptoms observed in Crohn's disease</b>","authors":["Hu W","Martinez Silgado A","Akkerman N","Limpens RWAL","Koning RI","Clevers H","Spaink HP"],"author_count":7,"year":2026,"published":"2026-02-13","venue":"Gut microbes","cited_by":3,"doi":"10.1080/19490976.2026.2630483","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1080/19490976.2026.2630483","archive_url":"https://space-kitty.com/papers/doi:10.1080/19490976.2026.2630483","updated":null,"pmid":"41688878","pmcid":"PMC12915785","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC12915785/pdf","abstract":"Crohn's disease (CD) is a chronic inflammatory bowel disease (IBD). Mycobacterium avium , which causes Johne's disease in ruminants, has been suggested as a potential CD trigger due to shared pathology, but early epithelial responses remain unclear. This study established a mouse small intestinal organoid (mSIO) model of M. avium infection to assess CD-related inflammation. Infected mSIOs were examined by confocal microscopy, block-face scanning electron microscopy, and macrophage co-culture to track bacterial localization and immune cell behavior. The data give unprecedent dynamic and super high resolution insights in the responses of gut cells to mycobacterial infection. RNA-seq with GSEA revealed strong induction of inflammatory genes and enrichment of pro-inflammatory pathways. Comparative analysis with CD-humanized mouse data showed overlapping gene expression and enrichment of the IBD signaling pathway. Notably, Mmp7 , which can be linked to epithelial remodeling and inflammation, was a common marker in both models. This study presents a robust mSIO model of M. avium infection that recapitulates features of CD-associated inflammation both with high-resolution imaging and transcriptomics and identifies Mmp7 as a potential molecular link between infection and CD-like pathology.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"<b>Responses of intestinal organoids to infection by</b> <i><b>Mycobacterium avium</b></i> <b>resemble symptoms observed in Crohn's disease</b>\" by Hu W, Martinez Silgado A, Akkerman N et al., https://doi.org/10.1080/19490976.2026.2630483, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"organoids","name":"Organoids & Organ-on-Chip","domain_slug":"biotech"}],"cited_in_dossiers":[]}