{"id":"doi:10.1093/nar/gkt489","kind":"article","title":"Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system","authors":["Fujiwara K","Katayama T","Nomura SM"],"author_count":3,"year":2013,"published":"2013-06-03","venue":"Nucleic acids research","cited_by":49,"doi":"10.1093/nar/gkt489","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1093/nar/gkt489","archive_url":"https://space-kitty.com/papers/doi:10.1093/nar/gkt489","updated":null,"pmid":"23737447","pmcid":"PMC3737561","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC3737561/pdf","abstract":"Replication of all living cells relies on the multirounds flow of the central dogma. Especially, expression of DNA replication proteins is a key step to circulate the processes of the central dogma. Here we achieved the entire sequential transcription-translation-replication process by autonomous expression of chromosomal DNA replication machineries from a reconstituted transcription-translation system (PURE system). We found that low temperature is essential to express a complex protein, DNA polymerase III, in a single tube using the PURE system. Addition of the 13 genes, encoding initiator, DNA helicase, helicase loader, RNA primase and DNA polymerase III to the PURE system gave rise to a DNA replication system by a coupling manner. An artificial genetic circuit demonstrated that the DNA produced as a result of the replication is able to provide genetic information for proteins, indicating the in vitro central dogma can sequentially undergo two rounds.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system\" by Fujiwara K, Katayama T, Nomura SM, https://doi.org/10.1093/nar/gkt489, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"synthetic-biology","name":"Synthetic Biology & Genome Engineering","domain_slug":"biotech"}],"cited_in_dossiers":[]}