{"id":"doi:10.1101/339531","kind":"article","title":"Whole-brain imaging and characterization of Drosophila brains based on one-, two-, and three-photon excitations","authors":["Kuo-Jen Hsu","Yen-Yin Lin","Ann‐Shyn Chiang","Shi‐Wei Chu"],"author_count":4,"year":2018,"published":"2018-06-05","venue":"bioRxiv (Cold Spring Harbor Laboratory)","cited_by":1,"doi":"10.1101/339531","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1101/339531","archive_url":"https://space-kitty.com/papers/doi:10.1101/339531","updated":null,"pmid":null,"pmcid":null,"openalex_id":"W2807292563","pdf_url":null,"abstract":"Abstract To study functional connectome, optical microscopy provides the advantages of in vivo observation, molecular specificity, high-speed acquisition, and sub-micrometer spatial resolution. Now, the most complete single-neuron-based anatomical connectome is built upon Drosophila ; thus it will be a milestone to achieve whole-brain observation with sub-cellular resolution in living Drosophila. Surprisingly, two-photon microscopy cannot penetrate through the 200-μm-thick brain, due to the extraordinarily strong aberration/scattering from tracheae. Here we achieve whole- Drosophila -brain observation by degassing the brain or by using three-photon microscopy at 1300-nm, while only the latter provides in vivo feasibility, reduced aberration/scattering and exceptional optical sectioning capability. Furthermore, by comparing one-photon (488-nm), two-photon (920-nm), and three-photon (1300-nm) excitations in the brain, we not only demonstrate first quantitative reduction of both scattering and aberration in trachea-filled tissues, but unravel that the contribution of aberration exceeds scattering at long wavelengths. Our work paves the way toward constructing functional connectome in a living Drosophila .","abstract_license":"CC-BY (publisher license, via OpenAlex)","license":"cc-by","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":["Advanced Fluorescence Microscopy Techniques"],"details":null,"provenance":"openalex","attribution":"Abstract of \"Whole-brain imaging and characterization of Drosophila brains based on one-, two-, and three-photon excitations\" by Kuo-Jen Hsu, Yen-Yin Lin, Ann‐Shyn Chiang et al., https://doi.org/10.1101/339531, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"connectomics","name":"Connectomics & Brain Mapping","domain_slug":"neuroscience"}],"cited_in_dossiers":[]}