{"id":"doi:10.1186/s12943-021-01431-6","kind":"review","title":"Application of the CRISPR/Cas9-based gene editing technique in basic research, diagnosis, and therapy of cancer","authors":["Huimin Zhang","Chunhong Qin","Changming An","Xiwang Zheng","Shuxin Wen","Wenjie Chen","Xianfang Liu","Zhenghua Lv","Ping–Chang Yang","Wei Xu","Wei Gao","Yongyan Wu"],"author_count":12,"year":2021,"published":"2021-10-01","venue":"Molecular Cancer","cited_by":249,"doi":"10.1186/s12943-021-01431-6","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1186/s12943-021-01431-6","archive_url":"https://space-kitty.com/papers/doi:10.1186/s12943-021-01431-6","updated":null,"pmid":"34598686","pmcid":null,"openalex_id":"W3204655553","pdf_url":"https://molecular-cancer.biomedcentral.com/track/pdf/10.1186/s12943-021-01431-6","abstract":"The 2020 Nobel Prize in Chemistry was awarded to Emmanuelle Charpentier and Jennifer Doudna for the development of the Clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease9 (CRISPR/Cas9) gene editing technology that provided new tools for precise gene editing. It is possible to target any genomic locus virtually using only a complex nuclease protein with short RNA as a site-specific endonuclease. Since cancer is caused by genomic changes in tumor cells, CRISPR/Cas9 can be used in the field of cancer research to edit genomes for exploration of the mechanisms of tumorigenesis and development. In recent years, the CRISPR/Cas9 system has been increasingly used in cancer research and treatment and remarkable results have been achieved. In this review, we introduced the mechanism and development of the CRISPR/Cas9-based gene editing system. Furthermore, we summarized current applications of this technique for basic research, diagnosis and therapy of cancer. Moreover, the potential applications of CRISPR/Cas9 in new emerging hotspots of oncology research were discussed, and the challenges and future directions were highlighted.","abstract_license":"CC-BY (publisher license, via OpenAlex)","license":"cc-by","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":["CRISPR and Genetic Engineering"],"details":null,"provenance":"openalex","attribution":"Abstract of \"Application of the CRISPR/Cas9-based gene editing technique in basic research, diagnosis, and therapy of cancer\" by Huimin Zhang, Chunhong Qin, Changming An et al., https://doi.org/10.1186/s12943-021-01431-6, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"gene-editing-therapies","name":"CRISPR & Gene-Editing Therapies","domain_slug":"medicine"}],"cited_in_dossiers":[]}