{"id":"doi:10.1186/s13059-018-1443-z","kind":"article","title":"Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion","authors":["Li C","Zong Y","Wang Y","Jin S","Zhang D","Song Q","Zhang R","Gao C"],"author_count":8,"year":2018,"published":"2018-05-29","venue":"Genome biology","cited_by":277,"doi":"10.1186/s13059-018-1443-z","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1186/s13059-018-1443-z","archive_url":"https://space-kitty.com/papers/doi:10.1186/s13059-018-1443-z","updated":null,"pmid":"29807545","pmcid":"PMC5972399","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC5972399/pdf","abstract":"Nucleotide base editors in plants have been limited to conversion of cytosine to thymine. Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine deaminase fused to the nickase CRISPR/Cas9, enabling A•T to G•C conversion at frequencies up to 7.5% in protoplasts and 59.1% in regenerated rice and wheat plants. An endogenous gene is also successfully modified through introducing a gain-of-function point mutation to directly produce an herbicide-tolerant rice plant. With this new adenine base editing system, it is now possible to precisely edit all base pairs, thus expanding the toolset for precise editing in plants.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion\" by Li C, Zong Y, Wang Y et al., https://doi.org/10.1186/s13059-018-1443-z, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"gene-editing-therapies","name":"CRISPR & Gene-Editing Therapies","domain_slug":"medicine"}],"cited_in_dossiers":[]}