{"id":"doi:10.1186/s13059-020-02170-5","kind":"article","title":"Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize","authors":["Jiang YY","Chai YP","Lu MH","Han XL","Lin Q","Zhang Y","Zhang Q","Zhou Y","Wang XC","Gao C","Chen QJ"],"author_count":11,"year":2020,"published":"2020-10-06","venue":"Genome biology","cited_by":170,"doi":"10.1186/s13059-020-02170-5","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.1186/s13059-020-02170-5","archive_url":"https://space-kitty.com/papers/doi:10.1186/s13059-020-02170-5","updated":null,"pmid":"33023639","pmcid":"PMC7541250","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC7541250/pdf","abstract":"Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize\" by Jiang YY, Chai YP, Lu MH et al., https://doi.org/10.1186/s13059-020-02170-5, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"gene-editing-therapies","name":"CRISPR & Gene-Editing Therapies","domain_slug":"medicine"}],"cited_in_dossiers":[]}