{"id":"doi:10.15252/msb.20198875","kind":"article","title":"Multiplex transcriptional characterizations across diverse bacterial species using cell-free systems","authors":["Yim SS","Johns NI","Park J","Gomes AL","McBee RM","Richardson M","Ronda C","Chen SP","Garenne D","Noireaux V","Wang HH"],"author_count":11,"year":2019,"published":"2019-08-01","venue":"Molecular systems biology","cited_by":66,"doi":"10.15252/msb.20198875","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.15252/msb.20198875","archive_url":"https://space-kitty.com/papers/doi:10.15252/msb.20198875","updated":null,"pmid":"31464371","pmcid":"PMC6692573","openalex_id":null,"pdf_url":"https://europepmc.org/articles/PMC6692573/pdf","abstract":"Cell-free expression systems enable rapid prototyping of genetic programs in vitro. However, current throughput of cell-free measurements is limited by the use of channel-limited fluorescent readouts. Here, we describe DNA Regulatory element Analysis by cell-Free Transcription and Sequencing (DRAFTS), a rapid and robust in vitro approach for multiplexed measurement of transcriptional activities from thousands of regulatory sequences in a single reaction. We employ this method in active cell lysates developed from ten diverse bacterial species. Interspecies analysis of transcriptional profiles from > 1,000 diverse regulatory sequences reveals functional differences in promoter activity that can be quantitatively modeled, providing a rich resource for tuning gene expression in diverse bacterial species. Finally, we examine the transcriptional capacities of dual-species hybrid lysates that can simultaneously harness gene expression properties of multiple organisms. We expect that this cell-free multiplex transcriptional measurement approach will improve genetic part prototyping in new bacterial chassis for synthetic biology.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"Multiplex transcriptional characterizations across diverse bacterial species using cell-free systems\" by Yim SS, Johns NI, Park J et al., https://doi.org/10.15252/msb.20198875, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"synthetic-biology","name":"Synthetic Biology & Genome Engineering","domain_slug":"biotech"}],"cited_in_dossiers":[]}