{"id":"doi:10.2144/000114249","kind":"article","title":"Preparation of amino acid mixtures for cell-free expression systems","authors":["Caschera F","Noireaux V"],"author_count":2,"year":2015,"published":"2015-01-01","venue":"BioTechniques","cited_by":38,"doi":"10.2144/000114249","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.2144/000114249","archive_url":"https://space-kitty.com/papers/doi:10.2144/000114249","updated":null,"pmid":"25605580","pmcid":null,"openalex_id":null,"pdf_url":null,"abstract":"Here we present a procedure for preparing amino acid mixtures--having both the desired composition and a physiological pH--at high concentrations for cell-free expression systems. Up to 2.1 mg/mL of active protein was synthesized in batch mode reactions with an all Escherichia coli cell-free expression system. Our method is fast, easy to execute, and economically advantageous compared to expensive commercial kits, making it useful for high-throughput experiments, incorporation of nonstandard amino acids, and cell-free metabolic engineering.","abstract_license":"CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)","license":"CC-BY","is_open_access":false,"retracted":false,"peer_reviewed":null,"categories":[],"details":null,"provenance":"europepmc","attribution":"Abstract of \"Preparation of amino acid mixtures for cell-free expression systems\" by Caschera F, Noireaux V, https://doi.org/10.2144/000114249, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"synthetic-biology","name":"Synthetic Biology & Genome Engineering","domain_slug":"biotech"}],"cited_in_dossiers":[]}