{"id":"doi:10.3389/fbioe.2023.1143157","kind":"review","title":"Off-target effects in CRISPR/Cas9 gene editing","authors":["Congting Guo","Xiaoteng Ma","Fei Gao","Yuxuan Guo"],"author_count":4,"year":2023,"published":"2023-03-09","venue":"Frontiers in Bioengineering and Biotechnology","cited_by":626,"doi":"10.3389/fbioe.2023.1143157","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.3389/fbioe.2023.1143157","archive_url":"https://space-kitty.com/papers/doi:10.3389/fbioe.2023.1143157","updated":null,"pmid":"36970624","pmcid":null,"openalex_id":"W4323666400","pdf_url":"https://www.frontiersin.org/articles/10.3389/fbioe.2023.1143157/pdf","abstract":"Gene editing stands for the methods to precisely make changes to a specific nucleic acid sequence. With the recent development of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system, gene editing has become efficient, convenient and programmable, leading to promising translational studies and clinical trials for both genetic and non-genetic diseases. A major concern in the applications of the CRISPR/Cas9 system is about its off-target effects, namely the deposition of unexpected, unwanted, or even adverse alterations to the genome. To date, many methods have been developed to nominate or detect the off-target sites of CRISPR/Cas9, which laid the basis for the successful upgrades of CRISPR/Cas9 derivatives with enhanced precision. In this review, we summarize these technological advancements and discuss about the current challenges in the management of off-target effects for future gene therapy.","abstract_license":"CC-BY (publisher license, via OpenAlex)","license":"cc-by","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":["CRISPR and Genetic Engineering"],"details":null,"provenance":"openalex","attribution":"Abstract of \"Off-target effects in CRISPR/Cas9 gene editing\" by Congting Guo, Xiaoteng Ma, Fei Gao et al., https://doi.org/10.3389/fbioe.2023.1143157, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"gene-editing-therapies","name":"CRISPR & Gene-Editing Therapies","domain_slug":"medicine"}],"cited_in_dossiers":[]}