{"id":"doi:10.7554/elife.107990","kind":"article","title":"Functional connectivity, structural connectivity, and inter-individual variability in Drosophila melanogaster","authors":["Takuto Okuno","Alexander Woodward","Hideyuki Okano","Junichi Hata"],"author_count":4,"year":2025,"published":"2025-10-09","venue":"eLife","cited_by":0,"doi":"10.7554/elife.107990","arxiv_id":null,"nct_id":null,"source_url":"https://doi.org/10.7554/elife.107990","archive_url":"https://space-kitty.com/papers/doi:10.7554/elife.107990","updated":null,"pmid":null,"pmcid":null,"openalex_id":"W4414986518","pdf_url":null,"abstract":"Clarifying the relationship between structure and function is important for understanding the brain. In Drosophila melanogaster, FlyEM and FlyWire electron microscopy-based connectome data and whole-brain calcium imaging data are available. We applied pre-processing methods from fMRI to whole-brain calcium imaging data and comprehensively investigated the optimal parameters. Then, we found that the FC-SC (functional and structural connectivity) correlation decreased linearly with region of interest count, and this trend was the same in flies and humans. We also developed a new, more robust method to quantify the degree of pre– and post-synaptic segregation and investigated this in the fly whole-brain. This revealed that many neurons have unsegregated synapses. We extracted highly unsegregated synapses and compared them with random-extracted null SC matrices. Their FC-SC correlation was significantly higher, indicating that these synapses contribute to FC well. Conversely, highly segregated-synapses showed significantly lower FC-SC correlation and contribute less to FC. Neurons with unsegregated synapses like non-spiking neurons are spread throughout the whole-brain, and they are thought to have a significant influence on FC.","abstract_license":"CC-BY (publisher license, via OpenAlex)","license":"cc-by","is_open_access":true,"retracted":false,"peer_reviewed":null,"categories":["Functional Brain Connectivity Studies"],"details":null,"provenance":"openalex","attribution":"Abstract of \"Functional connectivity, structural connectivity, and inter-individual variability in Drosophila melanogaster\" by Takuto Okuno, Alexander Woodward, Hideyuki Okano et al., https://doi.org/10.7554/elife.107990, used under its Creative Commons Attribution license (CC-BY); markup removed, otherwise unmodified.","topics":[{"slug":"connectomics","name":"Connectomics & Brain Mapping","domain_slug":"neuroscience"}],"cited_in_dossiers":[]}