# Preparation and Co-Culture of iPSC-Derived Dopaminergic Neurons and Astrocytes

Article · de Rus Jacquet A

- Archive id: doi:10.1002/cpcb.98
- Published: 2019-12-01
- Venue: Current protocols in cell biology
- DOI: https://doi.org/10.1002/cpcb.98
- PMID: 31763766
- PMCID: PMC9285934
- Citations: 33
- Source URL: https://doi.org/10.1002/cpcb.98
- PDF: https://europepmc.org/articles/PMC9285934/pdf
- Provenance: europepmc

## Abstract

Induced pluripotent stem cell (iPSC)-based models are powerful tools to study neurodegenerative diseases such as Parkinson's disease. The differentiation of patient-derived neurons and astrocytes allows investigation of the molecular mechanisms responsible for disease onset and development. In particular, these two cell types can be mono- or co-cultured to study the influence of cell-autonomous and non-cell-autonomous contributors to neurodegenerative diseases. We developed a streamlined procedure to produce high-quality/high-purity cultures of dopaminergic neurons and astrocytes that originate from the same population of midbrain floor-plate progenitors. This unit describes differentiation, quality control, culture parameters, and troubleshooting tips to ensure the highest quality and reproducibility of research results. © 2019 The Authors. Basic Protocol 1: Differentiation of iPSCs into midbrain-patterned neural progenitor cells Support Protocol: Quality control of neural progenitor cells Basic Protocol 2: Differentiation of neural progenitor cells into astrocytes Basic Protocol 3: Differentiation of neural progenitor cells into dopaminergic neurons Basic Protocol 4: Co-culture of iPSC-derived neurons and astrocytes.

_Abstract reproduced under CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)._

Topics: [Gene & Cell Therapy](https://space-kitty.com/topics/gene-cell-therapy)

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