# Extended Metabolic Biosensor Design for Dynamic Pathway Regulation of Cell Factories

Article · Boada Y, Vignoni A, Picó J, Carbonell P

- Archive id: doi:10.1016/j.isci.2020.101305
- Published: 2020-06-23
- Venue: iScience
- DOI: https://doi.org/10.1016/j.isci.2020.101305
- PMID: 32629420
- PMCID: PMC7334618
- Citations: 40
- Source URL: https://doi.org/10.1016/j.isci.2020.101305
- PDF: https://europepmc.org/articles/PMC7334618/pdf
- Provenance: europepmc

## Abstract

Transcription factor-based biosensors naturally occur in metabolic pathways to maintain cell growth and to provide a robust response to environmental fluctuations. Extended metabolic biosensors, i.e., the cascading of a bio-conversion pathway and a transcription factor (TF) responsive to the downstream effector metabolite, provide sensing capabilities beyond natural effectors for implementing context-aware synthetic genetic circuits and bio-observers. However, the engineering of such multi-step circuits is challenged by stability and robustness issues. In order to streamline the design of TF-based biosensors in metabolic pathways, here we investigate the response of a genetic circuit combining a TF-based extended metabolic biosensor with an antithetic integral circuit, a feedback controller that achieves robustness against environmental fluctuations. The dynamic response of an extended biosensor-based regulated flavonoid pathway is analyzed in order to address the issues of biosensor tuning of the regulated pathway under industrial biomanufacturing operating constraints.

_Abstract reproduced under CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)._

Topics: [Synthetic Biology & Genome Engineering](https://space-kitty.com/topics/synthetic-biology)

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