# Large-volume en-bloc staining for electron microscopy-based connectomics

Article · Hua Y, Laserstein P, Helmstaedter M

- Archive id: doi:10.1038/ncomms8923
- Published: 2015-08-03
- Venue: Nature communications
- DOI: https://doi.org/10.1038/ncomms8923
- PMID: 26235643
- PMCID: PMC4532871
- Citations: 255
- Source URL: https://doi.org/10.1038/ncomms8923
- PDF: https://europepmc.org/articles/PMC4532871/pdf
- Provenance: europepmc

## Abstract

Large-scale connectomics requires dense staining of neuronal tissue blocks for electron microscopy (EM). Here we report a large-volume dense en-bloc EM staining protocol that overcomes the staining gradients, which so far substantially limited the reconstructable volumes in three-dimensional (3D) EM. Our protocol provides densely reconstructable tissue blocks from mouse neocortex sized at least 1 mm in diameter. By relaxing the constraints on precise topographic sample targeting, it makes the correlated functional and structural analysis of neuronal circuits realistic.

_Abstract reproduced under CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)._

Topics: [Connectomics & Brain Mapping](https://space-kitty.com/topics/connectomics)

---
Space Kitty Archive · https://space-kitty.com · API: https://space-kitty.com/api/v1 · MCP: https://space-kitty.com/mcp · Premium: $0.20/request via x402 (Base Sepolia (testnet)). Metadata: arXiv (CC0), OpenAlex (CC0), Europe PMC (CC BY/CC0 works), ClinicalTrials.gov.