# Tuning the dynamic range of bacterial promoters regulated by ligand-inducible transcription factors

Article · Chen Y, Ho JML, Shis DL, Gupta C, Long J, Wagner DS, Ott W, Josić K, Bennett MR

- Archive id: doi:10.1038/s41467-017-02473-5
- Published: 2018-01-04
- Venue: Nature communications
- DOI: https://doi.org/10.1038/s41467-017-02473-5
- PMID: 29302024
- PMCID: PMC5754348
- Citations: 148
- Source URL: https://doi.org/10.1038/s41467-017-02473-5
- PDF: https://europepmc.org/articles/PMC5754348/pdf
- Provenance: europepmc

## Abstract

One challenge for synthetic biologists is the predictable tuning of genetic circuit regulatory components to elicit desired outputs. Gene expression driven by ligand-inducible transcription factor systems must exhibit the correct ON and OFF characteristics: appropriate activation and leakiness in the presence and absence of inducer, respectively. However, the dynamic range of a promoter (i.e., absolute difference between ON and OFF states) is difficult to control. We report a method that tunes the dynamic range of ligand-inducible promoters to achieve desired ON and OFF characteristics. We build combinatorial sets of AraC-and LasR-regulated promoters containing -10 and -35 sites from synthetic and Escherichia coli promoters. Four sequence combinations with diverse dynamic ranges were chosen to build multi-input transcriptional logic gates regulated by two and three ligand-inducible transcription factors (LacI, TetR, AraC, XylS, RhlR, LasR, and LuxR). This work enables predictable control over the dynamic range of regulatory components.

_Abstract reproduced under CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)._

Topics: [Synthetic Biology & Genome Engineering](https://space-kitty.com/topics/synthetic-biology)

---
Space Kitty Archive · https://space-kitty.com · API: https://space-kitty.com/api/v1 · MCP: https://space-kitty.com/mcp · Premium: $0.20/request via x402 (Base Sepolia (testnet)). Metadata: arXiv (CC0), OpenAlex (CC0), Europe PMC (CC BY/CC0 works), ClinicalTrials.gov.