# Automated microfluidic platform for dynamic and combinatorial drug screening of tumor organoids

Article · Schuster B, Junkin M, Kashaf SS, Romero-Calvo I, Kirby K, Matthews J, Weber CR, Rzhetsky A, White KP, Tay S

- Archive id: doi:10.1038/s41467-020-19058-4
- Published: 2020-10-19
- Venue: Nature communications
- DOI: https://doi.org/10.1038/s41467-020-19058-4
- PMID: 33077832
- PMCID: PMC7573629
- Citations: 311
- Source URL: https://doi.org/10.1038/s41467-020-19058-4
- PDF: https://europepmc.org/articles/PMC7573629/pdf
- Provenance: europepmc

## Abstract

Three-dimensional (3D) cell culture technologies, such as organoids, are physiologically relevant models for basic and clinical applications. Automated microfluidics offers advantages in high-throughput and precision analysis of cells but is not yet compatible with organoids. Here, we present an automated, high-throughput, microfluidic 3D organoid culture and analysis system to facilitate preclinical research and personalized therapies. Our system provides combinatorial and dynamic drug treatments to hundreds of cultures and enables real-time analysis of organoids. We validate our system by performing individual, combinatorial, and sequential drug screens on human-derived pancreatic tumor organoids. We observe significant differences in the response of individual patient-based organoids to drug treatments and find that temporally-modified drug treatments can be more effective than constant-dose monotherapy or combination therapy in vitro. This integrated platform advances organoids models to screen and mirror real patient treatment courses with potential to facilitate treatment decisions for personalized therapy.

_Abstract reproduced under CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)._

Topics: [Organoids & Organ-on-Chip](https://space-kitty.com/topics/organoids)

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