# PrimeDesign software for rapid and simplified design of prime editing guide RNAs

Article · Hsu JY, Grünewald J, Szalay R, Shih J, Anzalone AV, Lam KC, Shen MW, Petri K, Liu DR, Joung JK, Pinello L

- Archive id: doi:10.1038/s41467-021-21337-7
- Published: 2021-02-15
- Venue: Nature communications
- DOI: https://doi.org/10.1038/s41467-021-21337-7
- PMID: 33589617
- PMCID: PMC7884779
- Citations: 153
- Source URL: https://doi.org/10.1038/s41467-021-21337-7
- PDF: https://europepmc.org/articles/PMC7884779/pdf
- Provenance: europepmc

## Abstract

Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web application and command-line tool for the design of PE experiments. PrimeDesign can be used for single and combination editing applications, as well as genome-wide and saturation mutagenesis screens. Using PrimeDesign, we construct PrimeVar, a comprehensive and searchable database that includes candidate prime editing guide RNA (pegRNA) and nicking sgRNA (ngRNA) combinations for installing or correcting \>68,500 pathogenic human genetic variants from the ClinVar database. Finally, we use PrimeDesign to design pegRNAs/ngRNAs to install a variety of human pathogenic variants in human cells.

_Abstract reproduced under CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)._

Topics: [CRISPR & Gene-Editing Therapies](https://space-kitty.com/topics/gene-editing-therapies)

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