# Enhancement of prime editing via xrRNA motif-joined pegRNA

Article · Zhang G, Liu Y, Huang S, Qu S, Cheng D, Yao Y, Ji Q, Wang X, Huang X, Liu J

- Archive id: doi:10.1038/s41467-022-29507-x
- Published: 2022-04-06
- Venue: Nature communications
- DOI: https://doi.org/10.1038/s41467-022-29507-x
- PMID: 35387980
- PMCID: PMC8986804
- Citations: 96
- Source URL: https://doi.org/10.1038/s41467-022-29507-x
- PDF: https://europepmc.org/articles/PMC8986804/pdf
- Provenance: europepmc

## Abstract

The prime editors (PEs) have shown great promise for precise genome modification. However, their suboptimal efficiencies present a significant technical challenge. Here, by appending a viral exoribonuclease-resistant RNA motif (xrRNA) to the 3'-extended portion of pegRNAs for their increased resistance against degradation, we develop an upgraded PE platform (xrPE) with substantially enhanced editing efficiencies in multiple cell lines. A pan-target average enhancement of up to 3.1-, 4.5- and 2.5-fold in given cell types is observed for base conversions, small deletions, and small insertions, respectively. Additionally, xrPE exhibits comparable edit:indel ratios and similarly minimal off-target editing as the canonical PE3. Of note, parallel comparison of xrPE to the most recently developed epegRNA-based PE system shows their largely equivalent editing performances. Our study establishes a highly adaptable platform of improved PE that shall have broad implications.

_Abstract reproduced under CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)._

Topics: [CRISPR & Gene-Editing Therapies](https://space-kitty.com/topics/gene-editing-therapies)

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