# Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans

Article · Josephine Jüttner, Arnold Szabó, Brigitte Gross Scherf, Rei Morikawa, Santiago B. Rompani, Miguel Teixeira, Péter Hantz, Tamás Szikra, Federico Esposti, Cameron S. Cowan, Arjun Bharioke, Claudia P. Patino-Alvarez, Özkan Keles, Chiara N. Roth, Ákos Kusnyerik, Nadine Gerber-Hollbach, Thierry Azoulay, Dominik Hartl, Arnaud Regis Krebs, Dirk Schübeler, Rozina Hajdu, Ákos Lukáts, János Németh, Zoltan Z. Nagy, Kun-Chao Wu and 9 more

- Archive id: doi:10.1038/s41593-019-0431-2
- Published: 2019-07-08
- Venue: Nature Neuroscience
- DOI: https://doi.org/10.1038/s41593-019-0431-2
- PMID: 31285614
- Citations: 218
- Source URL: https://doi.org/10.1038/s41593-019-0431-2
- Provenance: openalex

## Abstract

Summary Targeting genes to specific neuronal or glial cell types is valuable both for understanding and for repairing brain circuits. Adeno-associated viral vectors (AAVs) are frequently used for gene delivery, but targeting expression to specific cell types is a challenge. We created a library of 230 AAVs, each with a different synthetic promoter designed using four independent strategies. We show that ~11% of these AAVs specifically target expression to neuronal and glial cell types in the mouse retina, mouse brain, non-human primate retina in vivo , and in the human retina in vitro . We demonstrate applications for recording, stimulation, and molecular characterization, as well as the intersectional and combinatorial labeling of cell types. These resources and approaches allow economic, fast, and efficient cell-type targeting in a variety of species, both for fundamental science and for gene therapy.

_Abstract reproduced under CC-BY (publisher license, via OpenAlex)._

Topics: [Gene & Cell Therapy](https://space-kitty.com/topics/gene-cell-therapy)

---
Space Kitty Archive · https://space-kitty.com · API: https://space-kitty.com/api/v1 · MCP: https://space-kitty.com/mcp · Premium: $0.20/request via x402 (Base Sepolia (testnet)). Metadata: arXiv (CC0), OpenAlex (CC0), Europe PMC (CC BY/CC0 works), ClinicalTrials.gov.