# Expanding the base editing scope in rice by using Cas9 variants

Article · Hua K, Tao X, Zhu JK

- Archive id: doi:10.1111/pbi.12993
- Published: 2018-10-05
- Venue: Plant biotechnology journal
- DOI: https://doi.org/10.1111/pbi.12993
- PMID: 30051586
- PMCID: PMC6335069
- Citations: 144
- Source URL: https://doi.org/10.1111/pbi.12993
- PDF: https://europepmc.org/articles/PMC6335069/pdf
- Provenance: europepmc

## Abstract

Base editing is a novel genome editing strategy that enables irreversible base conversion at target loci without the need for double stranded break induction or homology-directed repair. Here, we developed new adenine and cytosine base editors with engineered SpCas9 and SaCas9 variants that substantially expand the targetable sites in the rice genome. These new base editors can edit endogenous genes in the rice genome with various efficiencies. Moreover, we show that adenine and cytosine base editing can be simultaneously executed in rice. The new base editors described here will be useful in rice functional genomics research and will advance precision molecular breeding in crops.

_Abstract reproduced under CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)._

Topics: [CRISPR & Gene-Editing Therapies](https://space-kitty.com/topics/gene-editing-therapies)

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