# Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion

Article · Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C

- Archive id: doi:10.1186/s13059-018-1443-z
- Published: 2018-05-29
- Venue: Genome biology
- DOI: https://doi.org/10.1186/s13059-018-1443-z
- PMID: 29807545
- PMCID: PMC5972399
- Citations: 277
- Source URL: https://doi.org/10.1186/s13059-018-1443-z
- PDF: https://europepmc.org/articles/PMC5972399/pdf
- Provenance: europepmc

## Abstract

Nucleotide base editors in plants have been limited to conversion of cytosine to thymine. Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine deaminase fused to the nickase CRISPR/Cas9, enabling A•T to G•C conversion at frequencies up to 7.5% in protoplasts and 59.1% in regenerated rice and wheat plants. An endogenous gene is also successfully modified through introducing a gain-of-function point mutation to directly produce an herbicide-tolerant rice plant. With this new adenine base editing system, it is now possible to precisely edit all base pairs, thus expanding the toolset for precise editing in plants.

_Abstract reproduced under CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)._

Topics: [CRISPR & Gene-Editing Therapies](https://space-kitty.com/topics/gene-editing-therapies)

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