# Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

Article · Jiang YY, Chai YP, Lu MH, Han XL, Lin Q, Zhang Y, Zhang Q, Zhou Y, Wang XC, Gao C, Chen QJ

- Archive id: doi:10.1186/s13059-020-02170-5
- Published: 2020-10-06
- Venue: Genome biology
- DOI: https://doi.org/10.1186/s13059-020-02170-5
- PMID: 33023639
- PMCID: PMC7541250
- Citations: 170
- Source URL: https://doi.org/10.1186/s13059-020-02170-5
- PDF: https://europepmc.org/articles/PMC7541250/pdf
- Provenance: europepmc

## Abstract

Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors.

_Abstract reproduced under CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)._

Topics: [CRISPR & Gene-Editing Therapies](https://space-kitty.com/topics/gene-editing-therapies)

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