# Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor

Article · Park SJ, Jeong TY, Shin SK, Yoon DE, Lim SY, Kim SP, Choi J, Lee H, Hong JI, Ahn J, Seong JK, Kim K

- Archive id: doi:10.1186/s13059-021-02389-w
- Published: 2021-06-03
- Venue: Genome biology
- DOI: https://doi.org/10.1186/s13059-021-02389-w
- PMID: 34082781
- PMCID: PMC8173820
- Citations: 109
- Source URL: https://doi.org/10.1186/s13059-021-02389-w
- PDF: https://europepmc.org/articles/PMC8173820/pdf
- Provenance: europepmc

## Abstract

Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize and improve the efficiency of prime-editing. Herein, we introduce two strategies to improve the editing efficiency using proximal dead sgRNA and chromatin-modulating peptides. We used enhanced prime-editing to generate Igf2 mutant mice with editing frequencies of up to 47% and observed germline transmission, no off-target effects, and a dwarf phenotype. This improved prime-editing method can be efficiently applied to cell research and to generate mouse models.

_Abstract reproduced under CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)._

Topics: [CRISPR & Gene-Editing Therapies](https://space-kitty.com/topics/gene-editing-therapies)

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