# Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains

Article · Isaksson L, Mayzel M, Saline M, Pedersen A, Rosenlöw J, Brutscher B, Karlsson BG, Orekhov VY

- Archive id: doi:10.1371/journal.pone.0062947
- Published: 2013-05-07
- Venue: PloS one
- DOI: https://doi.org/10.1371/journal.pone.0062947
- PMID: 23667548
- PMCID: PMC3647075
- Citations: 41
- Source URL: https://doi.org/10.1371/journal.pone.0062947
- PDF: https://europepmc.org/articles/PMC3647075/pdf
- Provenance: europepmc

## Abstract

We present an integrated approach for efficient characterization of intrinsically disordered proteins. Batch cell-free expression, fast data acquisition, automated analysis, and statistical validation with data resampling have been combined for achieving cost-effective protein expression, and rapid automated backbone assignment. The new methodology is applied for characterization of five cytosolic domains from T- and B-cell receptors in solution.

_Abstract reproduced under CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)._

Topics: [Synthetic Biology & Genome Engineering](https://space-kitty.com/topics/synthetic-biology)

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