# Validation of Histopathology-Based Deep Learning Algorithms for Detection of Actionable Non-Small Cell Lung Cancer Biomarkers

Preprint · Rolfo C, Ofek E, Barak Y, Weidenfeld J, Haj RI, Molchanov Y, Loebel A, Braxton DR, Cupp JS, Yacobi R, Mayer C, Drago F, Avivi C, Paz-Yaacov N, Avinoam A, Zalach J, Dvir A, Gazy I, Peled N, Urban D, Bar J, Hershkoviz D, Barshack I

- Archive id: doi:10.21203/rs.3.rs-6675282/v1
- Published: 2025-06-12
- Venue: Research Square
- DOI: https://doi.org/10.21203/rs.3.rs-6675282/v1
- Citations: 0
- Source URL: https://doi.org/10.21203/rs.3.rs-6675282/v1
- Provenance: europepmc

## Abstract

Abstract Non-small cell lung cancer (NSCLC) patient management relies on molecular analysis to determine eligibility for targeted therapy. Furthermore, neoadjuvant immunotherapy is primarily suitable in the absence of specific genomic alterations. However, significant challenges such as suboptimal molecular testing and patients not receiving the most appropriate treatment remain. Here, we present AI classifiers for the identification of EGFR , ALK , BRAF and MET alterations directly from H&E-stained tissue using CanvOI 1.1, a digital pathology foundation model. Their performance was evaluated on an independent validation dataset of 968 NSCLC samples. The classifiers achieved AUCs of 0.87 for EGFR , 0.96 for ALK , 0.88 for BRAF and 0.83 for MET . Moreover, they demonstrated high accuracy in identifying cases lacking alterations. Our results highlight the potential of deep-learning tools for the detection of NSCLC biomarkers and specifically the identification of tumors without EGFR or ALK driver alterations, supporting more informed clinical decision-making.

_Abstract reproduced under CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)._

Topics: [AI in Clinical Medicine](https://space-kitty.com/topics/ai-medicine)

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