# Preparation of amino acid mixtures for cell-free expression systems

Article · Caschera F, Noireaux V

- Archive id: doi:10.2144/000114249
- Published: 2015-01-01
- Venue: BioTechniques
- DOI: https://doi.org/10.2144/000114249
- PMID: 25605580
- Citations: 38
- Source URL: https://doi.org/10.2144/000114249
- Provenance: europepmc

## Abstract

Here we present a procedure for preparing amino acid mixtures--having both the desired composition and a physiological pH--at high concentrations for cell-free expression systems. Up to 2.1 mg/mL of active protein was synthesized in batch mode reactions with an all Escherichia coli cell-free expression system. Our method is fast, easy to execute, and economically advantageous compared to expensive commercial kits, making it useful for high-throughput experiments, incorporation of nonstandard amino acids, and cell-free metabolic engineering.

_Abstract reproduced under CC-BY (article license as recorded by Europe PMC; abstract reproduced unmodified apart from markup removal)._

Topics: [Synthetic Biology & Genome Engineering](https://space-kitty.com/topics/synthetic-biology)

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