# Off-target effects in CRISPR/Cas9 gene editing

Review · Congting Guo, Xiaoteng Ma, Fei Gao, Yuxuan Guo

- Archive id: doi:10.3389/fbioe.2023.1143157
- Published: 2023-03-09
- Venue: Frontiers in Bioengineering and Biotechnology
- DOI: https://doi.org/10.3389/fbioe.2023.1143157
- PMID: 36970624
- Citations: 626
- Source URL: https://doi.org/10.3389/fbioe.2023.1143157
- PDF: https://www.frontiersin.org/articles/10.3389/fbioe.2023.1143157/pdf
- Provenance: openalex

## Abstract

Gene editing stands for the methods to precisely make changes to a specific nucleic acid sequence. With the recent development of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system, gene editing has become efficient, convenient and programmable, leading to promising translational studies and clinical trials for both genetic and non-genetic diseases. A major concern in the applications of the CRISPR/Cas9 system is about its off-target effects, namely the deposition of unexpected, unwanted, or even adverse alterations to the genome. To date, many methods have been developed to nominate or detect the off-target sites of CRISPR/Cas9, which laid the basis for the successful upgrades of CRISPR/Cas9 derivatives with enhanced precision. In this review, we summarize these technological advancements and discuss about the current challenges in the management of off-target effects for future gene therapy.

_Abstract reproduced under CC-BY (publisher license, via OpenAlex)._

Topics: [CRISPR & Gene-Editing Therapies](https://space-kitty.com/topics/gene-editing-therapies)

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