# Organoids & Organ-on-Chip Domain: Biotechnology & Life Sciences · 228 papers indexed · newest 2026-10-26 Brain and tissue organoids, assembloids, organ-on-chip platforms and their use in disease modeling and drug testing. Key concepts: organoid, organ-on-chip, assembloid ## Most influential - **[Cerebral organoids model human brain development and microcephaly](https://space-kitty.com/papers/doi:10.1038/nature12517)**. Madeline A. Lancaster, Magdalena Renner, Carol-Anne Martin et al. · 2013 · Nature (doi:10.1038/nature12517 · 5,369 citations). Source: - **[Organoid Models and Applications in Biomedical Research](https://space-kitty.com/papers/doi:10.1159/000433566)**. Christodoulos Xinaris, Valerio Brizi, Giuseppe Remuzzi · 2015 · The Nephron journals/Nephron journals (doi:10.1159/000433566 · 3,515 citations). Source: - **[Modeling Development and Disease with Organoids](https://space-kitty.com/papers/doi:10.1016/j.cell.2016.05.082)**. Hans C. Clevers · 2016 · Cell (doi:10.1016/j.cell.2016.05.082 · 3,196 citations). Source: - **[Distinct populations of inflammatory fibroblasts and myofibroblasts in pancreatic cancer](https://space-kitty.com/papers/doi:10.1084/jem.20162024)**. Öhlund D, Handly-Santana A, Biffi G et al. · 2017 · The Journal of experimental medicine (doi:10.1084/jem.20162024 · 2,274 citations). Source: > Pancreatic stellate cells (PSCs) differentiate into cancer-associated fibroblasts (CAFs) that produce desmoplastic stroma, thereby modulating disease progression and therapeutic response in pancreatic ductal adenocarcinoma (PDA). However, it is unknown whether CAFs uniformly carry out these tasks or if subtypes of CAFs with distinct phenotypes in PDA exist. We identified a CAF subpopulation with elevated expression of α-smooth muscle actin (αSMA) located immediately adjacent to neoplastic cells in mouse and human PDA tissue. We recapitulated this finding in co-cultures of murine PSCs and PDA o… > _Abstract excerpt by Öhlund D et al., CC-BY, https://doi.org/10.1084/jem.20162024; truncated._ - **[Organogenesis in a dish: Modeling development and disease using organoid technologies](https://space-kitty.com/papers/doi:10.1126/science.1247125)**. Madeline A. Lancaster, Juergen A. Knoblich · 2014 · Science (doi:10.1126/science.1247125 · 2,930 citations). Source: - **[Human organoids: model systems for human biology and medicine](https://space-kitty.com/papers/doi:10.1038/s41580-020-0259-3)**. Jihoon Kim, Bon‐Kyoung Koo, Juergen A. Knoblich · 2020 · Nature Reviews Molecular Cell Biology (doi:10.1038/s41580-020-0259-3 · 2,186 citations). Source: - **[SARS-CoV-2 B.1.617.2 Delta variant replication and immune evasion](https://space-kitty.com/papers/doi:10.1038/s41586-021-03944-y)**. Mlcochova P, Kemp SA, Dhar MS et al. · 2021 · Nature (doi:10.1038/s41586-021-03944-y · 1,142 citations). Source: > The B.1.617.2 (Delta) variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first identified in the state of Maharashtra in late 2020 and spread throughout India, outcompeting pre-existing lineages including B.1.617.1 (Kappa) and B.1.1.7 (Alpha) 1 . In vitro, B.1.617.2 is sixfold less sensitive to serum neutralizing antibodies from recovered individuals, and eightfold less sensitive to vaccine-elicited antibodies, compared with wild-type Wuhan-1 bearing D614G. Serum neutralizing titres against B.1.617.2 were lower in ChAdOx1 vaccinees than in BNT162b2 vaccinees. B.1.617.2… > _Abstract excerpt by Mlcochova P et al., CC-BY, https://doi.org/10.1038/s41586-021-03944-y; truncated._ - **[Organoid Models of Human and Mouse Ductal Pancreatic Cancer](https://space-kitty.com/papers/doi:10.1016/j.cell.2014.12.021)**. Sylvia F. Boj, Chang‐Il Hwang, Lindsey A. Baker et al. · 2014 · Cell (doi:10.1016/j.cell.2014.12.021 · 2,149 citations). Source: - **[Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure](https://space-kitty.com/papers/doi:10.1016/j.cell.2016.04.032)**. Xuyu Qian, Ha Nam Nguyen, Mingxi M. Song et al. · 2016 · Cell (doi:10.1016/j.cell.2016.04.032 · 2,127 citations). Source: - **[Organoids in cancer research](https://space-kitty.com/papers/doi:10.1038/s41568-018-0007-6)**. Jarno Drost, Hans C. Clevers · 2018 · Nature reviews. Cancer (doi:10.1038/s41568-018-0007-6 · 1,787 citations). Source: - **[Generation of cerebral organoids from human pluripotent stem cells](https://space-kitty.com/papers/doi:10.1038/nprot.2014.158)**. Madeline A. Lancaster, Juergen A. Knoblich · 2014 · Nature Protocols (doi:10.1038/nprot.2014.158 · 1,807 citations). Source: - **[Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis](https://space-kitty.com/papers/doi:10.1038/nature15695)**. Minoru Takasato, Pei Xuan Er, Han Sheng Chiu et al. · 2015 · Nature (doi:10.1038/nature15695 · 1,627 citations). Source: - **[Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver](https://space-kitty.com/papers/doi:10.1016/j.cell.2014.11.050)**. Meritxell Huch, Helmuth Gehart, Ruben van Boxtel et al. · 2014 · Cell (doi:10.1016/j.cell.2014.11.050 · 1,619 citations). Source: > Despite the enormous replication potential of the human liver, there are currently no culture systems available that sustain hepatocyte replication and/or function in vitro. We have shown previously that single mouse Lgr5+ liver stem cells can be expanded as epithelial organoids in vitro and can be differentiated into functional hepatocytes in vitro and in vivo. We now describe conditions allowing long-term expansion of adult bile duct-derived bipotent progenitor cells from human liver. The expanded cells are highly stable at the chromosome and structural level, while single base changes occur… > _Abstract excerpt by Meritxell Huch et al., CC-BY, https://doi.org/10.1016/j.cell.2014.11.050; truncated._ - **[Patient-derived lung cancer organoids as in vitro cancer models for therapeutic screening](https://space-kitty.com/papers/doi:10.1038/s41467-019-11867-6)**. Kim M, Mun H, Sung CO et al. · 2019 · Nature communications (doi:10.1038/s41467-019-11867-6 · 641 citations). Source: > Lung cancer shows substantial genetic and phenotypic heterogeneity across individuals, driving a need for personalised medicine. Here, we report lung cancer organoids and normal bronchial organoids established from patient tissues comprising five histological subtypes of lung cancer and non-neoplastic bronchial mucosa as in vitro models representing individual patient. The lung cancer organoids recapitulate the tissue architecture of the primary lung tumours and maintain the genomic alterations of the original tumours during long-term expansion in vitro. The normal bronchial organoids maintain… > _Abstract excerpt by Kim M et al., CC-BY, https://doi.org/10.1038/s41467-019-11867-6; truncated._ - **[In vitro generation of human pluripotent stem cell derived lung organoids](https://space-kitty.com/papers/doi:10.7554/elife.05098)**. Briana R. Dye, David R. Hill, Michael A.H. Ferguson et al. · 2015 · eLife (doi:10.7554/elife.05098 · 824 citations). Source: > Recent breakthroughs in 3-dimensional (3D) organoid cultures for many organ systems have led to new physiologically complex in vitro models to study human development and disease. Here, we report the step-wise differentiation of human pluripotent stem cells (hPSCs) (embryonic and induced) into lung organoids. By manipulating developmental signaling pathways hPSCs generate ventral-anterior foregut spheroids, which are then expanded into human lung organoids (HLOs). HLOs consist of epithelial and mesenchymal compartments of the lung, organized with structural features similar to the native lung.… > _Abstract excerpt by Briana R. Dye et al., CC-BY, https://doi.org/10.7554/elife.05098; truncated._ ## Newest - **[Patient-Derived Organoids from cryopreserved tissue can represent clinically relevant tools for drug testing in Biliary Cancer](https://space-kitty.com/papers/doi:10.1016/j.jhepr.2026.102083)**. Rae C, O'Rourke CJ, Tesini G et al. · 2026 · JHEP reports : innovation in hepatology (doi:10.1016/j.jhepr.2026.102083). Source: - **[High-fat diet-derived metabolite uridine diphosphate (UDP) impairs epithelial regeneration by P2Y \\6\\ -receptor signaling](https://space-kitty.com/papers/doi:10.1080/19490976.2026.2748160)**. Springer R, Claus P, Hörner M et al. · 2026 · Gut microbes (doi:10.1080/19490976.2026.2748160). Source: - **[Wireless bioelectronic control architectures for muscle-powered biohybrid robotic systems](https://space-kitty.com/papers/doi:10.1063/5.0339053)**. Tetsuka H, Hirano M · 2026 · APL bioengineering (doi:10.1063/5.0339053). Source: - **[Protocol for fabricating immune-regulatory bone organoids via plant-based bioinks](https://space-kitty.com/papers/doi:10.1016/j.isci.2026.117866)**. Wang T, Gao G, Zhang X et al. · 2026 · iScience (doi:10.1016/j.isci.2026.117866). Source: - **[Inhibition of WNT signaling sensitizes CRC cells to anti-tumor effects of retinoids: basis for new drug combinations in oncology](https://space-kitty.com/papers/doi:10.1080/15384047.2026.2732738)**. Hunsu VO, Facey COB, Opdenaker LM et al. · 2026 · Cancer biology & therapy (doi:10.1080/15384047.2026.2732738). Source: - **[Human induced pluripotent stem cells and organoid models in space for investigating severe pediatric metabolic and congenital disorders](https://space-kitty.com/papers/doi:10.1093/pcmedi/pbag028)**. Benedetti S, Štupica H, Zuccotti GV et al. · 2026 · Precision clinical medicine (doi:10.1093/pcmedi/pbag028). Source: - **[\Achyranthes bidentata\-functionalized bone organoids enhance bone defect repair](https://space-kitty.com/papers/doi:10.1016/j.mtbio.2026.103716)**. Lu C, Qin X, Lin Z et al. · 2026 · Materials today. Bio (doi:10.1016/j.mtbio.2026.103716). Source: - **[Systematic standardization of organ-on-a-chip under controlled flow conditions: Use case with Caki-1 and A549 cell lines](https://space-kitty.com/papers/doi:10.1016/j.mex.2026.104172)**. Balsa-Díaz A, Vázquez-Vázquez L, Ferreiro-Vila E et al. · 2026 · MethodsX (doi:10.1016/j.mex.2026.104172). Source: - **[Fibroblast-associated TPM2 links cell-matrix remodeling to EMT-Notch signaling and gemcitabine resistance in intrahepatic cholangiocarcinoma](https://space-kitty.com/papers/doi:10.1080/15384047.2026.2725344)**. Zhu Y, Qiao Z, Chen Z et al. · 2026 · Cancer biology & therapy (doi:10.1080/15384047.2026.2725344). Source: - **[Engineering high-fidelity bone organoids: Operational classification, multilineage crosstalk, biofabrication evidence, and translational validation](https://space-kitty.com/papers/doi:10.1016/j.mtbio.2026.103676)**. Huang Y, Zhang T, Chen S et al. · 2026 · Materials today. Bio (doi:10.1016/j.mtbio.2026.103676). Source: --- Space Kitty Archive · https://space-kitty.com · API: https://space-kitty.com/api/v1 · MCP: https://space-kitty.com/mcp · Premium: $0.20/request via x402 (Base Sepolia (testnet)). Metadata: arXiv (CC0), OpenAlex (CC0), Europe PMC (CC BY/CC0 works), ClinicalTrials.gov.